Cloning, purification, crystallization and preliminary crystallographic analysis of SecA from Enterococcus faecalis.

Identification of Polyketide Inhibitors Targeting 3-Dehydroquinate Dehydratase in the Shikimate Pathway of Enterococcus faecalis

Due to the emergence of resistance toward current antibiotics, there is a pressing need to develop the next generation of antibiotics as therapeutics against infectious and opportunistic diseases of microbial origins. The shikimate pathway is exclusive to microbes, plants and fungi, and hence is an attractive and logical target for development of antimicrobial therapeutics. The Gram-positive co...

Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

ElaGT is a glycosyltransferase from a marine Streptomyces species that is involved in the biosynthesis of elaiophylin. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of ElaGT are reported. The rod-shaped crystals belonged to space group P2(1)22, with unit-cell parameters a=66.7, b=131.7, c=224.6 Å, α=90, β=90, ...

Production, purification, crystallization and preliminary X-ray analysis of adeno-associated virus serotype 8.

Adeno-associated viruses (AAVs) are actively being developed for clinical gene-therapy applications and the efficiencies of the vectors could be significantly improved by a detailed understanding of their viral capsid structures and the structural determinants of their tissue-transduction interactions. AAV8 is approximately 80% identical to the more widely studied AAV2, but its liver-transducti...

Isolation and characterization of the Enterococcus faecalis strain isolated from red tilapia (Oreochromis hybrid) in Indonesia: A preliminary report

The number of fishes were conducted a series of bacteriological examinations to confirm the clinical symptoms that appeared and led to streptococcal infection. Results of the external examination from two moribund red tilapia found the hemorrhagic traces in some parts of the body such as the cranial area near the mouth, eyes, operculum, and some body parts and erosion on the tail, pectoral, and...

Cloning, expression, purification, crystallization and X-ray crystallographic analysis of glucuronic acid dehy­drogenase from Chromohalobacter salexigens. Addendum